Detail of Fig3A_WT
Project
SSBD:Repository
Title
The time-lapse images of observing in vitro-cultivated WT ovules expressing WOX2p::H2B-GFP and WOX2p::LTI-tdTomato
Description
The marker WOX2p::H2B-GFP to label the embryo nucleus of Arabidopsis and the marker WOX2p::LTI-tdTomato to label the plasma membrane (PM) showed that after the zygote division the apical cell of in vitro-cultivated WT ovules expands to take on a special shape before dividing vertically. The Arabidopsis lines expressing WOX2::H2B-GFP and WOX2p::LTI-tdTomato were original to the authors of this paper.
Release, Updated
2026-01-21
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
1.9 GB
Organism
Arabidopsis thaliana
Strain(s)
Col-0
Cell Line
nucleus plasma membrane
Datatype
-
Molecular Function (MF)
Biological Process (BP)
zygote elongation
Cellular Component (CC)
nucleus plasma membrane
Biological Imaging Method
spinning disk confocal microscopy (FBbi_00000253)
X scale
2.0 micrometer
Y scale
2.0 micrometer
Z scale
5.0 micrometer
T scale
10 minutes
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Tanaka S, Matsushita Y, Hanaki Y, Higaki T, Kamamoto N, Matsushita K, Higashiyama T, Fujimoto K, Ueda M. HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis. Curr Biol. 2024 Oct 21;34(20):4639-4649.e4.
Related paper(s)
Contact
Minako Ueda
, Tohoku University
, Graduate School of Life Sciences
, Laboratory of Plant Cell Dynamics
Contributors
OMERO Project