Detail of Fig1A_MbCD_withEGF_1
Project
SSBD:Repository
Title
Time-lapse TIRF images of EGFR-GFP on MbCD treated-CHO–K1 cells with EGF stimulation
Description
Time-lapse TIRF (total internal reflection fluorescence) images of EGFR-GFP on MbCD (methyl-beta-cyclodextrin) treated-CHO–K1 cells with EGF stimulation. Cells were administrated MbCD for 1 hour to deplete cholesterol from the plasma membrane.
Release, Updated
2025-11-28
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
33.8 MB
Datatype
-
Molecular Function (MF)
epidermal growth factor receptor activity
,
intracellular signal transduction
Biological Process (BP)
regulation of epidermal growth factor receptor signaling pathway
,
receptor clustering
Cellular Component (CC)
plasma membrane
,
receptor complex
Biological Imaging Method
evanescent wave microscopy
(
Fbbi:00000617
)
X scale
72 nanometer
Y scale
72 nanometer
Z scale
-
T scale
0.03 seconds, 30 frames per second
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Takayama M, Maeda S, Watanabe D, Takebayashi K, Hiroshima M, Ueda M. Cholesterol suppresses spontaneous activation of EGFR-mediated signal transduction. Biochem Biophys Res Commun . 2024 Apr 16:704:149673.
Related paper(s)
Contact
Michio Hiroshima, Masahiro Ueda
, Osaka University, Osaka University
, Graduate School of Science and Graduate School of Frontier Biosciences, Graduate School of Science and Graduate School of Frontier Biosciences
, Graduate School of Science and Graduate School of Frontier Biosciences, Graduate School of Science and Graduate School of Frontier Biosciences
Contributors
OMERO Project
Source