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Detail of Fig4_xenograft_plus1000_1

SSBD:Repository

Title

Z-series images of a BT-474 cell line-derived xenograft labeled with DyLight 650-Trastuzumab, PI, and CD31

Description

Z-series images of a xenograft derived from BT-474 human breast cancer cell line. The tumor xenograft was labeled with DyLight 650-Trastuzumab, propidium iodide, and CD31. The imaging was conducted with descSPIM, the desktop- equipped SPIM (selective plane illumination microscopy). The light sheet focus is 1000 um left side of the xenograft along the X axis.

Release, Updated

2025-11-28

Organism

Homo sapiens ( NCBI:txid9606 )

Strain(s)

Cell Line

BT-474 cell

Molecular Function (MF)

Biological Process (BP)

Cellular Component (CC)

nucleus plasma membrane

Biological Imaging Method

fluorescence microscopy ( Fbbi:00000246 )
selective plane illumination microscopy (SPIM)

X scale

3.5 micrometer

Y scale

3.5 micrometer

Z scale

10 micrometer

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA. descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nat Commun. 2024 Jun 12;15(1):4941.

Related paper(s)

Kohei Otomo, Takaki Omura, Yuki Nozawa, Steven J Edwards, Yukihiko Sato, Yuri Saito, Shigehiro Yagishita, Hitoshi Uchida, Yuki Watakabe, Kiyotada Naitou, Rin Yanai, Naruhiko Sahara, Satoshi Takagi, Ryohei Katayama, Yusuke Iwata, Toshiro Shiokawa, Yoku Hayakawa, Kensuke Otsuka, Haruko Watanabe-Takano, Yuka Haneda, Shigetomo Fukuhara, Miku Fujiwara, Takenobu Nii, Chikara Meno, Naoki Takeshita, Kenta Yashiro, Juan Marcelo Rosales Rocabado, Masaru Kaku, Tatsuya Yamada, Yumiko Oishi, Hiroyuki Koike, Yinglan Cheng, Keisuke Sekine, Jun-Ichiro Koga, Kaori Sugiyama, Kenichi Kimura, Fuyuki Karube, Hyeree Kim, Ichiro Manabe, Tomomi Nemoto, Kazuki Tainaka, Akinobu Hamada, Hjalmar Brismar, Etsuo A Susaki (2024) descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques., Nature communications, Volume 15, Number 1, pp. 4941

Published in 2024 Jun 12 (Electronic publication in June 12, 2024, midnight )

doi: 10.1038/s41467-024-49131-1
pmid: 38866781

(Abstract) Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.

(MeSH Terms)

Contact

Etsuo A Susaki , Juntendo University , Department of Biochemistry and Systems Biomedicine , Department of Biochemistry and Systems Biomedicine