Detail of Fig4_xenograft_plus500_2
Project
SSBD:Repository
Title
Z-series images of a BT-474 cell line-derived xenograft labeled with DyLight 650-Trastuzumab, PI, and CD31
Description
Z-series images of a xenograft derived from BT-474 human breast cancer cell line. The tumor xenograft was labeled with DyLight 650-Trastuzumab, propidium iodide, and CD31. The imaging was conducted with descSPIM, the desktop- equipped SPIM (selective plane illumination microscopy). The light sheet focus is 500 um left side of the xenograft along the X axis. This dataset is the result (angle1) of another result of the dataset (angle2), Fig4_ xenograft_plus500_1 , in which the images were measured from the oppsite side.
Release, Updated
2025-11-28
License
CC BY
Kind
Image data
File Formats
.tif
Data size
16.9 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
nucleus
,
plasma membrane
Biological Imaging Method
X scale
3.5 micrometer
Y scale
3.5 micrometer
Z scale
10 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA. descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nat Commun. 2024 Jun 12;15(1):4941.
Related paper(s)
Contact
Etsuo A Susaki
, Juntendo University
, Department of Biochemistry and Systems Biomedicine
, Department of Biochemistry and Systems Biomedicine
Contributors
OMERO Project