Detail of Fig3_brain_3Drender


Project
SSBD:Repository
Title
3D-rendered descSPIM image of Thy1-YFP-H mouse brain stained with propidium iodide
Description
Z-series images of Thy1-YFP-H mouse brain stained with propidium iodide, which were obtained with descSPIM, the desktop- equipped SPIM (selective plane illumination microscopy). This dataset is processed and combined data of other 8 datasets in this project.
Release, Updated
2025-11-28
License
CC BY
Kind
Image data
File Formats
NA
Data size
42.3 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
B6.Cg-Tg(Thy1-YFP)HJrs/J
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
nucleus , cytoplasm
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
selective plane illumination microscopy (SPIM)
X scale
3.5 micrometer
Y scale
3.5 micrometer
Z scale
10 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA. descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nat Commun. 2024 Jun 12;15(1):4941.
Related paper(s)

Contact
Etsuo A Susaki , Juntendo University , Department of Biochemistry and Systems Biomedicine , Department of Biochemistry and Systems Biomedicine
Contributors

OMERO Dataset
OMERO Project
Source