Description
Z-series images of Thy1-YFP-H mouse brain stained with propidium iodide, which were obtained with descSPIM, the desktop- equipped SPIM (selective plane illumination microscopy). This dataset contains the images of left and anterior region of the brain. The images were measured from dorsal side of the brain.
Summary of Methods
Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA. descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nat Commun. 2024 Jun 12;15(1):4941.
Contact
Etsuo A Susaki
, Juntendo University
, Department of Biochemistry and Systems Biomedicine
, Department of Biochemistry and Systems Biomedicine