Detail of Fig1C_NogChord_ctrl
Project
SSBD:Repository
Title
Z-series images of immotile cilia without Noggin and Chordin double-treatment
Description
Z-series images of immotile cilia expressing NDE2-hsp-Pkd2-Venus without Noggin and Chordin double- treatment. The immotile cilia in mouse embryo at 0-2 somite stages were immunostained with Pkd2::Venus and acetylated tubulin.
Release, Updated
2025-11-28
License
CC BY
Kind
Image data
File Formats
.czi
Data size
3.3 GB
Organism
Mus musculus
(
NCBI:txid10090
)
Strain(s)
B6;B6C3(129)-Tg(NDE-hsp-Pkd2/Venus)1Hmd
Cell Line
-
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
non-motile cilium
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
0.168 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Katoh TA, Lange T, Nakajima Y, Yashiro K, Okada Y, Hamada H. BMP4 regulates asymmetric Pkd2 distribution in mouse nodal immotile cilia and ciliary mechanosensing required for left-right determination. Dev Dyn. 2024 Jul 10.
Related paper(s)
Takanobu A Katoh, Tim Lange, Yoshiro Nakajima, Kenta Yashiro, Yasushi Okada, Hiroshi Hamada (2024) BMP4 regulates asymmetric Pkd2 distribution in mouse nodal immotile cilia and ciliary mechanosensing required for left-right determination., Developmental dynamics : an official publication of the American Association of Anatomists
Published in 2024 Jul 10 (Electronic publication in July 10, 2024, midnight )
- doi: 10.1002/dvdy.727
-
pmid: 38984461
(Abstract) BACKGROUND: Mouse nodal immotile cilia mechanically sense the bending direction for left-right (L-R) determination and activate the left-side-specific signaling cascade, leading to increased Nodal activity. Asymmetric distribution of Pkd2, a crucial channel for L-R determination, on immotile cilia has been reported recently. However, the causal relationship between the asymmetric Pkd2 distribution and direction-dependent flow sensing is not well understood. Furthermore, the underlying molecular mechanism directing this asymmetric Pkd2 distribution remains unclear. RESULTS: The effects of several recombinant proteins and inhibitors on the Pkd2 distribution were analyzed using super-resolution microscopy. Notably, bone morphogenetic protein 4 (BMP4) affected the Pkd2 distribution. Additionally, three-dimensional manipulation of nodal immotile cilia using optical tweezers revealed that excess BMP4 caused defects in the mechanosensing ability of the cilia. CONCLUSIONS: Experimental data together with model calculations suggest that BMP4 regulates the asymmetric distribution of Pkd2 in nodal immotile cilia, thereby affecting the ability of these cilia to sense the bending direction for L-R determination. This study, for the first time, provides insight into the relationship between the asymmetric protein distribution in cilia and their function.
Contact
Contributors
OMERO Project
Source