Z-series images of mouse brain stained with SYTOX-Green
Description
Z-series SYTOX-Green images of mouse brain obtained by AMATERAS2. The colonally cutted 1.5 mm brain section was chemically cleared using CUBIC, and the cell nuclei were stained with SYTOX-Green. The images were obtained with 4x magnification system, and the background intensity was removed by the computational sectioning.
spinning disk confocal microscopy
(
Fbbi:00000253
)
AMATERAS-2c
X scale
0.55 micrometer
Y scale
0.55 micrometer
Z scale
4 micrometer
T scale
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Image Acquisition
Experiment type
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Microscope type
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Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Ichimura T, Kakizuka T, Taniguchi Y, Ejima S, Sato Y, Itano K, Seiriki K, Hashimoto H, Sugawara K, Itoga H, Onami S, Nagai T. Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo. Elife. 2025 Feb 3;13:RP93633
Related paper(s)
Contact
Taro Ichimura, Takeharu Nagai
, Osaka University, Osaka University
, Institute for Open and Transdisciplinary Research Initiatives, Institute for Open and Transdisciplinary Research Initiatives
, Institute for Open and Transdisciplinary Research Initiatives, Institute for Open and Transdisciplinary Research Initiatives