Detail of Fig5_Brain_2fold


Project
SSBD:Repository
Title
Z-series images of mouse brain stained with SYTOX-Green
Description
Z-series SYTOX-Green images of mouse brain obtained by AMATERAS2. The colonally cutted 1.5 mm-thick brain section was chemically cleared using CUBIC, and the cell nuclei were stained with SYTOX-Green. The images were obtained with 2x magnification system, and the background intensity was removed by the computational sectioning.
Release, Updated
2025-11-26
License
CC BY
Kind
Image data
File Formats
.tif
Data size
42.9 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
C57BL/6J
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
nucleus
Biological Imaging Method
spinning disk confocal microscopy ( Fbbi:00000253 )
AMATERAS-2c
X scale
1.1 micrometer
Y scale
1.1 micrometer
Z scale
4 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Ichimura T, Kakizuka T, Taniguchi Y, Ejima S, Sato Y, Itano K, Seiriki K, Hashimoto H, Sugawara K, Itoga H, Onami S, Nagai T. Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo. Elife. 2025 Feb 3;13:RP93633
Related paper(s)

Contact
Taro Ichimura, Takeharu Nagai , Osaka University, Osaka University , Institute for Open and Transdisciplinary Research Initiatives, Institute for Open and Transdisciplinary Research Initiatives , Institute for Open and Transdisciplinary Research Initiatives, Institute for Open and Transdisciplinary Research Initiatives
Contributors
Taishi Kakizuka, Yoshitsugu Taniguchi, Satoshi Ejima, Yuki Sato, Kaoru Seiriki, Hitoshi Hashimoto

OMERO Dataset
OMERO Project
Source