Detail of Fig4F_VB_Mock
Project
SSBD:Repository
Title
Z-series images of the vascular bed after infection with mock virus
Description
Z-series images of the vascular bed after infection with mock virus. Bronchial organoids and vascular bed composed of HUVECs (Human Umbilical Vein Endothelial Cells) were co-cultured in a special 3D microfluidic device. The virus was applied to the side of bronchial organoids, but not to the vascular bed side. Channel1: ICAM1, Channel2: Alexa Fluor 647 labeled phalloidin, Channel3: DAPI, Channel4: GFP, Channel4: Differential interference image
Release, Updated
2025-11-26
License
CC BY
Kind
Image data
File Formats
.oir
Data size
630.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
viral process
Cellular Component (CC)
nucleus
actin cytoskeleton
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
1.24 micrometer
Y scale
1.24 micrometer
Z scale
5.18 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto K, Kameda Y, Nagano Y, Deguchi S, Yamamoto T, Krol RP, Gee P, Matsumura Y, Okamoto T, Nagao M, Takayama K, Yokokawa R. SARS-CoV-2-induced disruption of a vascular bed in a microphysiological system caused by type-I interferon from bronchial organoids. Lab Chip . 2024 Aug 6;24(16):3863-3879.
Related paper(s)
Kazuya Fujimoto, Yoshikazu Kameda, Yuta Nagano, Sayaka Deguchi, Takuya Yamamoto, Rafal P Krol, Peter Gee, Yasufumi Matsumura, Toru Okamoto, Miki Nagao, Kazuo Takayama, Ryuji Yokokawa (2024) SARS-CoV-2-induced disruption of a vascular bed in a microphysiological system caused by type-I interferon from bronchial organoids., Lab on a chip
Published in 2024 Jan 22 (Electronic publication in Jan. 22, 2024, midnight )
- doi: 10.1039/d3lc00768e
-
pmid: 38252025
(Abstract) Blood vessels show various COVID-19-related conditions including thrombosis and cytokine propagation. Existing in vitro blood vessel models cannot represent the consequent changes in the vascular structure or determine the initial infection site, making it difficult to evaluate how epithelial and endothelial tissues are damaged. Here, we developed a microphysiological system (MPS) that co-culture the bronchial organoids and the vascular bed to analyze infection site and interactions. In this system, virus-infected organoids caused damage in vascular structure. However, vasculature was not damaged or infected when the virus was directly introduced to vascular bed. The knockout of interferon-related genes and inhibition of the JAK/STAT pathway reduced the vascular damage, indicating the protective effect of interferon response suppression. The results demonstrate selective infection of bronchial epithelial cells and vascular damage by cytokines and also indicate the applicability of MPS to investigate how the infection influences vascular structure and functions.
Contact
Ryuji Yokokawa, Kazuo Takayama
, Kyoto University, Kyoto University
Contributors
OMERO Project
Source