Detail of Video12_COX8a_reagent_1
Project
SSBD:Repository
Title
Time-lapse images of COX8a=mStayGold in HeLa cells in series application of histamine, cyproheptadine, and ionomycin.
Description
Time-lapse images of COX8a=mStayGold in HeLa cells in series application of histamine, cyproheptadine, and ionomycin.. Histamine, cyproheptadine, and ionomycin were applied at 1 minute, 2.5 minutes, and 4 minutes, respectively.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.vsi
Data size
3.1 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cellular response to histamine
,
cellular response to ionomycin
Cellular Component (CC)
mitochondrion
Biological Imaging Method
spinning disk confocal microscopy
(
Fbbi:00000253
)
single-beam laser-scanning confocal microscopy (LSCM)
single-beam laser-scanning confocal microscopy (LSCM)
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
-
T scale
0.4 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project