Detail of Video11_COX8a
Project
SSBD:Repository
Title
Time-lapse images of COX8a=mStayGold in HeLa cells, showing extremely dynamic and complicated mitochondrial membrane
Description
HeLa cells expressing COX8a=mStayGold, which visualize inner mitochondrial membrane dynamics, were imaged by SDSRM (SpinSR10). Single-plane images were acquired continuously at 2.41 frames/second (exposure time: 400 ms). This data highlights a mobile mitochondrion.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.vsi
Data size
2.0 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
Biological Imaging Method
spinning disk confocal microscopy
(
Fbbi:00000253
)
single-beam laser-scanning confocal microscopy (LSCM)
single-beam laser-scanning confocal microscopy (LSCM)
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
-
T scale
0.41 second, 2.41 frames/second
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project