Detail of Fig5c_COX8a
Project
SSBD:Repository
Title
Time-lapse lattice SIM images of COX8a=mStayGold in HeLa cells
Description
Time-lapse lattice SIM images of COX8a=mStayGold in HeLa cells. To visualize inner mitochondrial membranes, the authors fused mStayGold to the C terminus of subunit CoxVIIIa of cytochrome c oxidase (COX8a=mStayGold). Cells were 3D scanned with a z step size of 0.11 micrometer over an axial range of 2.08 micrometer by lattice SIM.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.czi
Data size
648.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
Biological Imaging Method
structured illumination microscopy (SIM) (Fbbi:
00000332)
lattice SIM
00000332)
lattice SIM
X scale
0.03 micrometer
Y scale
0.03 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project
Source