Detail of ExtFig8_Ftractin_LatA


Project
SSBD:Repository
Title
Time-lapse images of F-tractin=mStayGold in COS-7 cells treated with latrunculin A.
Description
Time-lapse images of F-tractin=mStayGold, which visualize filamentous actin, in COS-7 cells after treatment with latrunculin A.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.vsi
Data size
9.8 GB

Organism
Chlorocebus pygerythrus ( NCBI:txid60710 )
Strain(s)
-
Cell Line
COS-7 cell

Datatype
-
Molecular Function (MF)
Biological Process (BP)
negative regulation of actin filament polymerization
Cellular Component (CC)
actin cytoskeleton
Biological Imaging Method
spinning disk confocal microscopy ( Fbbi:00000253 )
single-beam laser-scanning confocal microscopy (LSCM)
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
-
T scale
0.41 second, 2.41 frames/second

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)

Contact
Atsushi Miyawaki , RIKEN , Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science , Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors

OMERO Dataset
OMERO Project
Source