Detail of SupplFig16_GianCreg_Tub
Project
SSBD:Repository
Title
Time-lapse images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging
Description
Time-lapse images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging in a single channel. COS-7 cells were cotransfected with td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin. A fine 3D reconstruction of the whole Golgi apparatus with a microtubule network was generated by volumetric imaging (z-step, 0.216 micrometer; z-range, 1.94 micrometer) using the lattice SIM.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.czi
Data size
88.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Golgi apparatus
Biological Imaging Method
structured illumination microscopy (SIM) (Fbbi:
00000332)
lattice SIM
00000332)
lattice SIM
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Ryoko Ando, Satoshi Shimozono, Hideo Ago, Masatoshi Takagi, Mayu Sugiyama, Hiroshi Kurokawa, Masahiko Hirano, Yusuke Niino, Go Ueno, Fumiyoshi Ishidate, Takahiro Fujiwara, Yasushi Okada, Masaki Yamamoto, Atsushi Miyawaki (2023) StayGold variants for molecular fusion and membrane-targeting applications., Nature methods
Published in 2023 Nov 30 (Electronic publication in Nov. 30, 2023, midnight )
- doi: 10.1038/s41592-023-02085-6
-
pmid: 38036853
(Abstract) Although StayGold is a bright and highly photostable fluorescent protein, its propensity for obligate dimer formation may hinder applications in molecular fusion and membrane targeting. To attain monovalent as well as bright and photostable labeling, we engineered tandem dimers of StayGold to promote dispersibility. On the basis of the crystal structure of this fluorescent protein, we disrupted the dimerization to generate a monomeric variant that offers improved photostability and brightness compared to StayGold. We applied the new monovalent StayGold tools to live-cell imaging experiments using spinning-disk laser-scanning confocal microscopy or structured illumination microscopy. We achieved cell-wide, high-spatiotemporal resolution and sustained imaging of dynamic subcellular events, including the targeting of endogenous condensin I to mitotic chromosomes, the movement of the Golgi apparatus and its membranous derivatives along microtubule networks, the distribution of cortical filamentous actin and the remolding of cristae membranes within mobile mitochondria.
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project
Source