Detail of SupplFig16_GianCreg_Tub
Project
SSBD:Repository
Title
Time-lapse images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging
Description
Time-lapse images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging in a single channel. COS-7 cells were cotransfected with td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin. A fine 3D reconstruction of the whole Golgi apparatus with a microtubule network was generated by volumetric imaging (z-step, 0.216 micrometer; z-range, 1.94 micrometer) using the lattice SIM.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.czi
Data size
88.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Golgi apparatus
Biological Imaging Method
structured illumination microscopy (SIM) (Fbbi:
00000332)
lattice SIM
00000332)
lattice SIM
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project