Detail of Video6_GianCreg_Tub
Project
SSBD:Repository
Title
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging
Description
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells, which were obtained by dual-target imaging in a single channel. To visualize the Golgi apparatus, the authors fused StayGold through c4 to the N terminus of a region (amino acids 3,131-3,259) of human giantin (td5StayGold(c4)=GianCreg).
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.vsi
Data size
1.6 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Golgi apparatus
Biological Imaging Method
structured illumination microscopy (SIM) (Fbbi:
00000332)
lattice SIM
00000332)
lattice SIM
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
0.5 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project