Detail of Video5_GianCreg_1
Project
SSBD:Repository
Title
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg in HeLa cells
Description
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg in HeLa cells, which visualize Golgi membrane dynamics. To visualize the Golgi apparatus, the authors fused StayGold through c4 to the N terminus of a region (amino acids 3,131-3,259) of human giantin (td5StayGold(c4)=GianCreg).
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.vsi
Data size
4.4 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Golgi organization
Cellular Component (CC)
Golgi apparatus
Biological Imaging Method
spinning disk confocal microscopy
(
Fbbi:00000253
)
single-beam laser-scanning confocal microscopy (LSCM)
single-beam laser-scanning confocal microscopy (LSCM)
X scale
0.04 micrometer
Y scale
0.04 micrometer
Z scale
0.5 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project