Detail of ExtFig6a_chromosome_fast
Project
SSBD:Repository
Title
Time-lapse images of CAP-H-td5oxStayGold and SiR-DNA-labeled chromosomes in HCT116 cells
Description
Time-lapse images of CAP-H-td5oxStayGold and SiR-DNA-labeled chromosomes in HCT116 cells. With CRISPR-Cas9 genome-editing technique, a subunit of condensin I (CAP-H) was endogenously tagged at the C terminus with td5oxStayGold (CAP-H-td5oxStayGold) in the HCT116 cells.
Release, Updated
2025-11-26
License
CC BY 4.0
Kind
Image data
File Formats
.vsi
Data size
46.0 GB
Datatype
-
Molecular Function (MF)
regulate the chromosome assembly and segregation
Biological Process (BP)
chromosome organization
Cellular Component (CC)
nucleus
,
chromosome
Biological Imaging Method
spinning disk confocal microscopy
(
Fbbi:00000253
)
single-beam laser-scanning confocal microscopy (LSCM)
single-beam laser-scanning confocal microscopy (LSCM)
X scale
0.13 micrometer
Y scale
0.13 micrometer
Z scale
-
T scale
1 second
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.
Related paper(s)
Contact
Atsushi Miyawaki
, RIKEN
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
, Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Contributors
OMERO Project