SSBD:database

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Detail of Fig7E_ACTKDonGel_0204_2

Project

292-Katsuta-StressFiber

SSBD:Repository

ssbd-repos-000292

Title

Phase contrast images of alpha-actinin knockdown C2C12 cell migration on gel

Description

Z-series and time-lapse images of actinin knockdown C2C12 cell dynamics for up to 240 minutes. C2C12 cell were cultured on polyacrylamide gel substrates.

Release, Updated

2025-04-10

License

CC BY

Kind

Image data

File Formats

.ics, .ids

Data size

160.2 MB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

Cell Line

C2C12 cell

Datatype

Molecular Function (MF)

Biological Process (BP)

cell migration

Cellular Component (CC)

Biological Imaging Method

confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )

X scale

0.44 micrometer/pixel

Y scale

0.44 micrometer/pixel

Z scale

2 micrometer

T scale

10 minutes

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Katsuta H, Okuda S, Nagayama K, Machiyama H, Kidoaki S, Kato M, Sokabe M, Miyata T, Hirata H. Actin crosslinking by alpha-actinin averts viscous dissipation of myosin force transmission in stress fibers. iScience. 2023 Feb1;26(3):106090.