Detail of Fig5D_Video6_actKO
Project
SSBD:Repository
Title
Time-lapse images of actin-GFP FRAP on stress fibers in alpha-actinin knockdown C2C12 cells
Description
Fluorescence recovery after photobleaching (FRAP) experiments of alpha-actinin knockdown C2C12 cells. Stress fibers in C2C12 cells expressing GFP-tagged actin were locally photobleached with 405 nm laser for 0.5 sec with 100 percent of the laser power (the size of bleached area was 1.6 x 3.6 mm), and fluo- rescence images were acquired every 0.5 sec for 1 min with 3-10 percent of the laser power.
Release, Updated
2025-04-10
License
CC BY
Kind
Image data
File Formats
.nd2
Data size
16.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
actin nucleation
Cellular Component (CC)
cytoskeleton
,
stress fiber
Biological Imaging Method
X scale
0.224 micrometer/pixel
Y scale
0.224 micrometer/pixel
Z scale
-
T scale
0.5 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Katsuta H, Okuda S, Nagayama K, Machiyama H, Kidoaki S, Kato M, Sokabe M, Miyata T, Hirata H. Actin crosslinking by alpha-actinin averts viscous dissipation of myosin force transmission in stress fibers. iScience. 2023 Feb1;26(3):106090.
Related paper(s)
Contact
Hiroaki Hirata
, Kanazawwa Institute of Technology
Contributors
Hiroki Katsuta, Satoru Okuda, Kazuaki Nagayama, Hiroaki Machiyama, Hiroaki Hirata
OMERO Project