Detail of Fig1F_actKO


Project
SSBD:Repository
Title
Time-lapse images of LifeAct-mCherry and myosin IIA-GFP in alpha-actinin knockdown cells
Description
Time-lapse images of LifeAct-mCherry and myosin IIA-GFP in alpha-actinin knockdown cells, showing the dynamics of stress fiber.
Release, Updated
2025-04-10
License
CC BY
Kind
Image data
File Formats
.nd2
Data size
84.4 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
C2C12 cell

Datatype
-
Molecular Function (MF)
Biological Process (BP)
regulation of stress fiber assembly
Cellular Component (CC)
cytoskeleton , stress fiber
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
X scale
0.171 micrometer/pixel
Y scale
0.171 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Katsuta H, Okuda S, Nagayama K, Machiyama H, Kidoaki S, Kato M, Sokabe M, Miyata T, Hirata H. Actin crosslinking by alpha-actinin averts viscous dissipation of myosin force transmission in stress fibers. iScience. 2023 Feb1;26(3):106090.
Related paper(s)

Contact
Hiroaki Hirata , Kanazawwa Institute of Technology
Contributors
Hiroki Katsuta, Satoru Okuda, Kazuaki Nagayama, Hiroaki Machiyama, Hiroaki Hirata

OMERO Dataset
OMERO Project
Source