Detail of Fig2C_Ca_conditionWT_9


Project
SSBD:Repository
Title
Time-lapse calcium imaging of ASEL in conditioned WT C-elegans
Description
Time-lapse calcium imaging of ASEL, a gustatory neuron, in conditioned wild type (WT) C-elegans. The WT worm was conditioned to be attracted to glucose beforehand. The autors used YC2.60 as a calcium indicator, and measured fluorescence intensity ratio of YFP to CFP. Left-half are CFP images, and Right half are YFP images. The glucose concentration was switched from 15 to 0 mM at 50 seconds and then returned to 15 mM at 100 seconds.
Release, Updated
2025-04-10
License
CC BY
Kind
Image data
File Formats
tif
Data size
122.3 MB

Organism
Caenorhabditis elegans ( NCBI:txid6239 )
Strain(s)
JN2103
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to chemical ( GO:0042221 )
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
time lapse microscopy ( Fbbi:00000249 )
X scale
0.4 micrometer
Y scale
0.4 micrometer
Z scale
-
T scale
0.5 seconds

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Tomioka M, Umemura Y, Ueoka Y, Chin R, Katae K, Uchiyama C, Ike Y, Iino Y. Antagonistic regulation of salt and sugar chemotaxis plasticity by a single chemosensory neuron in Caenorhabditis elegans. PLoS Genet. 2023 Sep 5;19(9):e1010637. PLoS Genet. 2023 Sep 5;19(9):e1010637.
Related paper(s)

Contact
Masahiro Tomioka, Yuichi Iino , National Institude of Occupational Safety and Health, Japan, The University of Tokyo , Division of Industrial Toxicology, Department of Biological Sciences, Graduate School of Science , Division of Industrial Toxicology, Department of Biological Sciences, Graduate School of Science
Contributors
Risshun Chin

OMERO Dataset
OMERO Project
Source