Time-lapse calcium imaging of ASEL in conditioned WT C-elegans
Description
Time-lapse calcium imaging of ASEL, a gustatory neuron, in conditioned wild type (WT) C-elegans. The WT worm was conditioned to be attracted to glucose beforehand. The autors used YC2.60 as a calcium indicator, and measured fluorescence intensity ratio of YFP to CFP. Left-half are CFP images, and Right half are YFP images. The glucose concentration was switched from 15 to 0 mM at 50 seconds and then returned to 15 mM at 100 seconds.
Tomioka M, Umemura Y, Ueoka Y, Chin R, Katae K, Uchiyama C, Ike Y, Iino Y. Antagonistic regulation of salt and sugar chemotaxis plasticity by a single chemosensory neuron in Caenorhabditis elegans. PLoS Genet. 2023 Sep 5;19(9):e1010637. PLoS Genet. 2023 Sep 5;19(9):e1010637.
Related paper(s)
Contact
Masahiro Tomioka, Yuichi Iino
, National Institude of Occupational Safety and Health, Japan, The University of Tokyo
, Division of Industrial Toxicology, Department of Biological Sciences, Graduate School of Science
, Division of Industrial Toxicology, Department of Biological Sciences, Graduate School of Science