Detail of FigS2A_FGFinternalize


Project
SSBD:Repository
Title
Time-lapse images of bright field, FGF1-Alexa488, and FGF1-pHrodo in an isolated lung epithelium embedded within the Matrigel
Description
Time-lapse images of bright field, FGF1-Alexa488, and FGF1-pHrodo in an isolated lung epithelium embedded within the Matrigel. To visualize internalized FGF1, pHrodo-labeled FGF1 and Alexa488-labeled FGF1 were added to the culture ex vivo. Channel1; bright field, Channel2; FGF1-Alexa488, Channel3; FGF1-pHrodo
Release, Updated
2025-02-13
License
CC BY
Kind
Image data
File Formats
.oib
Data size
444.3 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
Slc:ICR
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
lung lobe formation , signal transduction ,
Cellular Component (CC)
cytosol
Biological Imaging Method
two-photon laser scanning microscopy ( Fbbi:00000254 )
X scale
0.397 micrometer
Y scale
0.397 micrometer
Z scale
-
T scale
1 minute

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Hirashima T and Matsuda M. ERK-mediated curvature feedback regulates branching morphogenesis in lung epithelial tissue. Curr Biol. 2024 Feb 26;34(4):683-696.e6.
Related paper(s)

Contact
Tsuyoshi Hirashima , National University of Singapore , Mechanobiology Institute , Mechanobiology Institute
Contributors

OMERO Dataset
OMERO Project
Source