Detail of Fig2b_DNAunderwind
Project
SSBD:Repository
Title
Z-series Lattice-SIM images of DAPI, bio-psoralen and RNAPIIS2P in nucleus
Description
Z-series Lattice-SIM images of DAPI, bio-psoralen and RNAPIIS2P in nucleus. The underwound DNA was labeled with biotinylated psoralen, which preferentially binds to underwound DNA rather than relaxed DNA, before cell fixation. Channel1;RNAPIIS2P (RNA polymerase II phosphorylated at serine 2), Chennel2;DAPI, Channel3;psoralen
Release, Updated
2025-01-31
License
CC BY
Kind
Image data
File Formats
.czi
Data size
840.3 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
nucleus
Biological Imaging Method
Lattice-pattern structured illumination microscopy
X scale
0.05 micrometer
Y scale
0.05 micrometer
Z scale
0.40 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fukute J, Maki K, Adachi T. The nucleolar shell provides anchoring sites for DNA untwisting. Commun Biol. 2024 Jan 23;7(1):83.
Related paper(s)
Contact
Koichiro Maki
, Kyoto University
, Laboratory of Cellular and Molecular Biomechanics, Department of Mammalian Regulatory Network, Graduate School of Biostudies
, Laboratory of Cellular and Molecular Biomechanics, Department of Mammalian Regulatory Network, Graduate School of Biostudies
Contributors
Jumpei Fukute, Koichiro Maki, Taiji Adachi
OMERO Project