Detail of FigS8_mVenus-TERT_3
Project
SSBD:Repository
Title
Time-lapse images showing the translocation of mVenus-TERT in HeLa cells during apoptosis induced by oxidative stress
Description
Time-lapse images showing the translocation of mVenus-TERT (Telomerase reverse transcriptase) in HeLa cells during apoptosis induced by oxidative stress.This dataset is composed of 12 series of datasets, each of which is time-lapse image of 5 x 5 tiling data. Channel2; bright field, Channel4; mVenus, Channel5; MitoTracker Deep Red FM, Channe6; SYTOX Blue. The 12 series consist of 4 genotypes, and 2 experimental conditions. Genotypes: Series1-Series3; without transfection, Series4-Series6; mVenus-TERT transfection, Series7-Series9; mVenus-mutant TERT (R3ER6E) transfection, Series10-Series12; mVenus-mutant TERT (Y707F) transfection. Experimental conditions: Series1, 4, 7, 10; without oxidative stress, Series2, 3, 5, 6, 8, 9, 11, 12; with oxidative stress.
Release, Updated
2024-12-24
License
CC BY
Kind
Image data
File Formats
.nd2
Data size
141.9 GB
Datatype
-
Molecular Function (MF)
telomerase activity
Biological Process (BP)
regulation of apoptotic process
Cellular Component (CC)
mitochondrion
,
nucleus
Biological Imaging Method
X scale
0.216 micrometer
Y scale
0.216 micrometer
Z scale
-
T scale
1 hour
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ebata H, Shima T, Iizuka R, Uemura S. Accumulation of TERT in mitochondria exerts two opposing effects on apoptosis. FEBS Open Bio. 2023, Sep;13(9) :1667-1682.
Related paper(s)
Contact
Tomohiro Shima, Sotaro Uemura
, The University of Tokyo, The University of Tokyo
, Department of Biological Sciences, Graduate School of Science, Department of Biological Sciences, Graduate School of Science
, Department of Biological Sciences, Graduate School of Science, Department of Biological Sciences, Graduate School of Science
Contributors
Hiroshi Ebata
OMERO Project