SSBD:database

Detail of Fig2_FigS2_TERT_3

Project

366-Ebata-TERTlocalize

SSBD:Repository

ssbd-repos-000366

Title

Time-lapse images of HeLa cells with or without expressing the TERT constructs during apoptosis

Description

Time-lapse images of HeLa cells with or without expressing the TERT (Telomerase reverse transcriptase) constructs during apoptosis induced by oxidative stress. This dataset is composed of 16 series of dataset; series1-series16, each of which is 3-day long time-lapse image of 7 x 7 tiling data. Channel2; bright field, Channel4; SYTOX Orange, Channel5; merge. The 16 series consist of 4 genotypes and 4 experimental conditions. Genotypes: Series1-Series4; without transfection, Series5-Series8; TERT transfection, Series9-Series12; mutant TERT (R3ER6E) transfection, Series13-Series16; mutant TERT (Y707F) transfection. Experimental conditions: Series1, 5, 9, 13; SYTOX Orange was used as an apotosis marker, Series2, 6, 10, 14; YO-PRO-1 was used as an apotosis marker, Series3, 7, 11, 15; SYTOX Orange imaging with treatment of Z-VAD-fmk, Series 4, 8, 12, 16; SYTOX Orange imaging with treatment of GSK'872.

Release, Updated

2024-12-24

License

CC BY

Kind

Image data

File Formats

.nd2

Data size

549.5 GB

Organism

Homo sapiens ( NCBI:txid9606 )

Strain(s)

Cell Line

HeLa cell

Datatype

Molecular Function (MF)

telomerase activity

Biological Process (BP)

regulation of apoptotic process

Cellular Component (CC)

mitochondrion , nucleus

Biological Imaging Method

fluorescence microscopy ( Fbbi:00000246 )
time lapse microscopy ( Fbbi:00000249 )

X scale

0.324 micrometer

Y scale

0.324 micrometer

Z scale

T scale

1 hour

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Ebata H, Shima T, Iizuka R, Uemura S. Accumulation of TERT in mitochondria exerts two opposing effects on apoptosis. FEBS Open Bio. 2023, Sep;13(9) :1667-1682.