Detail of 100ms_ThT_1.5x_40x_ND8_1_2_Pos0
Project
SSBD:Repository
Title
Fluorescence images of thioflavin-T with mCherry-Nter (N-terminal region of Argonaute2) in the absence of seeds of Nter aggregates.
Description
Fluorescence images of thioflavin-T, an amyloid detection reagent, with mCherry-Nter (N-terminal region of Argonaute2) in the absence of seeds of Nter aggregates. In the absence of the seeds, the ThT fluorescence intensity showed an initial lag phase of 300 min and then reached a plateau at 800 min after mixing monomeric Nter with ThT in aggregation buffer.
Release, Updated
2024-12-23
License
CC BY
Kind
Image data
File Formats
.tif
Data size
722.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
protein-containing complex assembly
(
GO:0065003
)
Cellular Component (CC)
Biological Imaging Method
X scale
0.108 micrometer/pixel
Y scale
0.108 micrometer/pixel
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Narita H, et. al. BMC Biol. 2023 Apr 19;21(1):78.
Related paper(s)
Contact
Tomohiro Shima, Sotaro Uemura
, University of Tokyo, University of Tokyo
, Department of Biological Sciences, Graduate School of Science , Department of Biological Sciences, Graduate School of Science
Contributors
Haruka Narita
OMERO Project
Source