Detail of Fig5c_TensionCaluculinA
Project
SSBD:Repository
Title
Time-lapse images of tension sensor in VSMCs isolated from the aorta before and after stimulation with calyculin A
Description
Time-lapse images of actinin tension sensor in VSMCs (Vascular smooth muscle cells) isolated from the aorta before and after stimulation with calyculin A. Decrease in energy transfer from EGFP to mCherry reflects the increase in cell tension. Calyculin A was added between 21-22 min.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.czi
Data size
631.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to chemical
Cellular Component (CC)
cytoskeleton
Biological Imaging Method
FRET
(
Fbbi:00000367
)
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
X scale
0.264 micrometer/pixel
Y scale
0.264 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
1 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Wang J, Maeda E, Tsujimura Y, Abe T, Kiyonari H, Kitaguchi T, Yokota H, Matsumoto T. In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor. Sci Rep. 2023 Dec 20;13(1):22729.
Related paper(s)
Contact
Takeo Matsumoto
, Nagoya University
, Biomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering
, Biomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering
Contributors
Junfeng Wang, Yuki Tsujimura, Takaya Abe, Hiroshi Kiyonari, Tetsuya Kitaguchi
OMERO Project
Source