Detail of Fig4EG_MTelongation_3
Project
SSBD:Repository
Title
Time-series images of x-ray fiber diffraction measuring axial pitch of GDP-MT before and after addition of K11C or AMPPMP
Description
Time-series images of x-ray fiber diffraction measuring axial pitch of GDP-MT (GDP-microtubules) before and after addition of K11C or AMPPMP. K11C is a mutant KIF5C, the L11-alpha4 junction was replaced with the corresponding sequence of KIF1A. A synchrotron radiation x-ray beam at BL45XU beamline of SPring-8 (Japan Synchrotron Radiation Research Institute) irradiated the oriented microtubules. K11C was added after time00001, and AMPPMP was applied after time00011.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
tif
Data size
189.0 MB
Organism
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Strain(s)
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Cell Line
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Datatype
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Molecular Function (MF)
Biological Process (BP)
microtubule bundle maintenance
Cellular Component (CC)
Biological Imaging Method
X scale
0.017 nanometer^{-1}/pixel
Y scale
0.017 nanometer^{-1}/pixel
Z scale
-
T scale
15 seconds per time interval
Image Acquisition
Experiment type
-
Microscope type
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Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N. Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport. J Cell Biol. 2018 Dec 3;217(12):4164-4183.
Related paper(s)
Contact
Yasushi Okada, Nobutaka Hirokawa
, RIKEN BDR, The University of Tokyo
, Laboratory for Cell Polarity Regulation, Department of Cell Biology and Anatomy, Graduate School of Medicine
, Laboratory for Cell Polarity Regulation, Department of Cell Biology and Anatomy, Graduate School of Medicine
Contributors
Tomohiro Shima, Sotaro Uemura, Yasushi Okada
OMERO Project