Detail of Fig1DE_KIF5C_GTP-MT
Project
SSBD:Repository
Title
Time-lapse images of KIF5C moving on GTP-MT
Description
Time-lapse images of KIF5C moving on GMPCPP (guanylyl-alpha, beta-methylene-diphosphonate)-microtubules. GMPCPP is a slowly hydrolyzable GTP analogue, and GMPCPP-microtubules were used as GTP conformation of microtubules. The dimeric KIF5C (constitutive active recombinant: K560) runs on every GMPCPP-microtubule in the field. KIF5C was genetically fused with eGFP.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
tif
Data size
506.7 MB
Organism
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Strain(s)
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Cell Line
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Datatype
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Molecular Function (MF)
microtubule motor activity
Biological Process (BP)
microtubule-based transport
Cellular Component (CC)
Biological Imaging Method
X scale
60 nanometer/pixel
Y scale
60 nanometer/pixel
Z scale
-
T scale
0.1 seconds per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N. Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport. J Cell Biol. 2018 Dec 3;217(12):4164-4183.
Related paper(s)
Contact
Yasushi Okada, Nobutaka Hirokawa
, RIKEN BDR, The University of Tokyo
, Laboratory for Cell Polarity Regulation, Department of Cell Biology and Anatomy, Graduate School of Medicine
, Laboratory for Cell Polarity Regulation, Department of Cell Biology and Anatomy, Graduate School of Medicine
Contributors
Tomohiro Shima, Sotaro Uemura, Yasushi Okada
OMERO Project