Detail of Fig2D_pretreat
Project
Title
Immunofluorescence images for persistent infection model of SARS-CoV-2 before treatment
Description
Immunofluorescence images for persistent infection model of SARS-CoV-2 before treatment. cTnT (green), S protein (red), and DAPI (blue).
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.tif
Data size
2.4 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
viral process
(
GO:0016032
)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
566 micrometer/pixel
Y scale
566 micrometer/pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Murata K. et al. Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model. iScience. 2023 Dec 22;27(1):108641.
Related paper(s)
Kozue Murata, Akiko Makino, Keizo Tomonaga, Hidetoshi Masumoto (2024) Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model., iScience, Volume 27, Number 1, pp. 108641
Published in 2024 Jan 19 (Electronic publication in Dec. 22, 2023, midnight )
- doi: 10.1016/j.isci.2023.108641
-
pmid: 38299028
(Abstract) Patients with chronic cardiomyopathy may have persistent viral infections in their hearts, particularly with SARS-CoV-2, which targets the ACE2 receptor highly expressed in human hearts. This raises concerns about a potential global heart failure pandemic stemming from COVID-19, an SARS-CoV-2 pandemic in near future. Although faced with this healthcare caveat, there is limited research on persistent viral heart infections, and no models have been established. In this study, we created an SARS-CoV-2 persistent infection model using human iPS cell-derived cardiac microtissues (CMTs). Mild infections sustained viral presence without significant dysfunction for a month, indicating persistent infection. However, when exposed to hypoxic conditions mimicking ischemic heart diseases, cardiac function deteriorated alongside intracellular SARS-CoV-2 reactivation in cardiomyocytes and disrupted vascular network formation. This study demonstrates that SARS-CoV-2 persistently infects the heart opportunistically causing cardiac dysfunction triggered by detrimental stimuli such as ischemia, potentially predicting a post COVID-19 era heart failure pandemic.
Contact
Keizo Tomonaga, Hidetoshi Masumoto
, Kyoto University, RIKEN Center for Biosystems Dynamics Research
, LabLaboratory of RNA Viruses, Department of Virus Research, Institute for Life and Medical Sciencesoratory for Developmental Dynamics, Clinical Translational Research Program
, LabLaboratory of RNA Viruses, Department of Virus Research, Institute for Life and Medical Sciencesoratory for Developmental Dynamics, Clinical Translational Research Program
Contributors
Kozue Murata
OMERO Project