Detail of MovieS3_MitoShape_oryzalin
Project
Title
Live-cell image of mitochondria in Arabidopsis thaliana zygotes in the presence of polymerization inhibitor for microtubules, oryzalin
Description
The green-fluorescent reporter visualizes mitochondria (DD45p, mt-GFP) and the red-fluorescent reporter labels nucleus (EC1p, H2B-tdTomato and DD22p, H2B- mCherry). In the presence of polymerization inhibitor for microtubules (1 micromolar oryzalin), which blocks proper spindle formation and thus prolongs the cell division period, the mitochondria kept their dotted shape during this period.
Release, Updated
2024-12-14
License
CC-BY
Kind
Image data
File Formats
.nd2
Data size
4.0 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
mitochondrion distribution
(
GO:0048311
)
zygote asymmetric cell division
(
GO:0010070
)
Cellular Component (CC)
mitochondrion
(
GO:0005739
)
Biological Imaging Method
X scale
0.168 micrometer/pixel
Y scale
0.168 micrometer/pixel
Z scale
1 micrometer/slice
T scale
20 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Kimata Y, et. al. Quant Plant Biol. 2020 Nov 30;1:e3.
Related paper(s)
Yusuke Kimata, Takumi Higaki, Daisuke Kurihara, Naoe Ando, Hikari Matsumoto, Tetsuya Higashiyama, Minako Ueda (2020) Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes., Quantitative plant biology, Volume 1, pp. e3
Published in 2020 (Electronic publication in Nov. 30, 2020, midnight )
- doi: 10.1017/qpb.2020.4
-
pmid: 37077329
(Abstract) The zygote is the first cell of a multicellular organism. In most angiosperms, the zygote divides asymmetrically to produce an embryo-precursor apical cell and a supporting basal cell. Zygotic division should properly segregate symbiotic organelles, because they cannot be synthesized de novo. In this study, we revealed the real-time dynamics of the principle source of ATP biogenesis, mitochondria, in Arabidopsis thaliana zygotes using live-cell observations and image quantifications. In the zygote, the mitochondria formed the extended structure associated with the longitudinal array of actin filaments (F-actins) and were polarly distributed along the apical-basal axis. The mitochondria were then temporally fragmented during zygotic division, and the resulting apical cells inherited mitochondria at higher concentration compared to the basal cells. Further observation of postembryonic organs showed that these mitochondrial behaviours are characteristic of the zygote. Overall, our results showed that the zygote has spatiotemporal regulation that unequally distributes the mitochondria.
Contact
Minako Ueda
, Tohoku University
, Graduate School of Life Sciences
, Laboratory of Plant Cell Dynamics
Contributors
Yusuke Kimata, Naoe Ando, Hikari Matsumoto
OMERO Project
Source