Detail of MovieS3_MitoShape_oryzalin
Project
SSBD:Repository
Title
Live-cell image of mitochondria in Arabidopsis thaliana zygotes in the presence of polymerization inhibitor for microtubules, oryzalin
Description
The green-fluorescent reporter visualizes mitochondria (DD45p, mt-GFP) and the red-fluorescent reporter labels nucleus (EC1p, H2B-tdTomato and DD22p, H2B- mCherry). In the presence of polymerization inhibitor for microtubules (1 micromolar oryzalin), which blocks proper spindle formation and thus prolongs the cell division period, the mitochondria kept their dotted shape during this period.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.nd2
Data size
4.0 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
mitochondrion distribution
(
GO:0048311
)
,
zygote asymmetric cell division
(
GO:0010070
)
Cellular Component (CC)
mitochondrion
(
GO:0005739
)
Biological Imaging Method
X scale
0.168 micrometer/pixel
Y scale
0.168 micrometer/pixel
Z scale
1 micrometer/slice
T scale
20 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Kimata Y, et. al. Quant Plant Biol. 2020 Nov 30;1:e3.
Related paper(s)
Contact
Minako Ueda
, Tohoku University
, Graduate School of Life Sciences
, Laboratory of Plant Cell Dynamics
Contributors
Yusuke Kimata, Naoe Ando, Hikari Matsumoto
OMERO Project
Source