Detail of MovieS2_MitoShape_CellDiv


Project
SSBD:Repository
Title
Live-cell image of mitochondria in Arabidopsis thaliana zygotes before and after the zygotic division
Description
The green-fluorescent reporter visualizes mitochondria (DD45p, mt-GFP) and the red-fluorescent reporter labels nucleus (EC1p, H2B-tdTomato and DD22p, H2B- mCherry). Many dotted mitochondria were observed instead of the longitudinal filaments during cell division, and the filamentous structures were detected again after zygotic division was completed.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.nd2
Data size
8.7 GB

Organism
Arabidopsis thaliana ( NCBI:txid3702 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
mitochondrion distribution ( GO:0048311 ) , zygote asymmetric cell division ( GO:0010070 )
Cellular Component (CC)
mitochondrion ( GO:0005739 )
Biological Imaging Method
two-photon laser scanning microscopy ( Fbbi:00000254 )
time lapse microscopy ( Fbbi:00000249 )
X scale
0.126 micrometer/pixel
Y scale
0.126 micrometer/pixel
Z scale
1 micrometer/slice
T scale
10 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Kimata Y, et. al. Quant Plant Biol. 2020 Nov 30;1:e3.
Related paper(s)

Contact
Minako Ueda , Tohoku University , Graduate School of Life Sciences , Laboratory of Plant Cell Dynamics
Contributors
Yusuke Kimata, Naoe Ando, Hikari Matsumoto

OMERO Dataset
OMERO Project
Source