Detail of Fig4K_cobl_Noco
Project
Title
Time-series images of cell dynamics in WT cells before and after nocodazole treatment
Description
Time-series images of cell dynamics in WT cells before (control) and after nocodazole treatment (20min, 40min, 60min). The image of Cobl in WT cells after wash out of nocodazole is also shown. Channel1 is ZO-1, Channel2 is F-actin and Channel3 is cobl.
Release, Updated
2024-12-14
License
CC-BY
Kind
Image data
File Formats
.tif
Data size
30.0 MB
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain(s)
-
Cell Line
Eph4 mouse mammary gland epithelial cell
Datatype
-
Molecular Function (MF)
molecular condensate scaffold activity
(
GO:0140693
)
Biological Process (BP)
actin filament depolymerization
(
GO:0030042
)
Cellular Component (CC)
cytosol
(
GO:0005829
)
Biological Imaging Method
X scale
0.04 micrometer/pixel
Y scale
0.04 micrometer/pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Tsukita K, et. al. Sci Adv. 2023 Feb 15;9(7):eadf6358.
Related paper(s)
Kazuto Tsukita, Manabu Kitamata, Hiroka Kashihara, Tomoki Yano, Ikuko Fujiwara, Timothy F Day, Tatsuya Katsuno, Jaewon Kim, Fumiko Takenaga, Hiroo Tanaka, Sungsu Park, Makoto Miyata, Hitomi Watanabe, Gen Kondoh, Ryosuke Takahashi, Atsushi Tamura, Sachiko Tsukita (2023) Phase separation of an actin nucleator by junctional microtubules regulates epithelial function., Science advances, Volume 9, Number 7, pp. eadf6358
Published in 2023 Feb 15 (Electronic publication in Feb. 15, 2023, midnight )
- doi: 10.1126/sciadv.adf6358
-
pmid: 36791197
(Abstract) Liquid-liquid phase separation (LLPS) is involved in various dynamic biological phenomena. In epithelial cells, dynamic regulation of junctional actin filaments tethered to the apical junctional complex (AJC) is critical for maintaining internal homeostasis against external perturbations; however, the role of LLPS in this process remains unknown. Here, after identifying a multifunctional actin nucleator, cordon bleu (Cobl), as an AJC-enriched microtubule-associated protein, we conducted comprehensive in vitro and in vivo analyses. We found that apical microtubules promoted LLPS of Cobl at the AJC, and Cobl actin assembly activity increased upon LLPS. Thus, microtubules spatiotemporally regulated junctional actin assembly for epithelial morphogenesis and paracellular barriers. Collectively, these findings established that LLPS of the actin nucleator Cobl mediated dynamic microtubule-actin cross-talk in junctions, which fine-tuned the epithelial barrier.(MeSH Terms)
Contact
Atsushi Tamura, Sachiko Tsukita
, Teikyo University, Teikyo University
, Advanced Comprehensive Research Organization, Advanced Comprehensive Research Organization
Contributors
Kazuto Tsukita
OMERO Project
Source