Detail of Fig6F_Acidify-by-light_siVDAC2
Project
SSBD:Repository
Title
Time-lapse images of siVDAC2 A431 cells transfected with expression vectors for CRY2-MiCy-FYVE and TOM20-iRFPCIB1 incubated with AcidiFluor-Dx exposed to 488-nm light.
Description
Time-lapse images of siVDAC2 A431 cells transfected with expression vectors for CRY2-MiCy-FYVE and TOM20-iRFPCIB1 incubated with AcidiFluor-Dx exposed to 488-nm light.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.tif
Data size
336.5 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
endosomal lumen acidification
(
GO:0048388
)
Cellular Component (CC)
early endosome
(
GO:0005769
)
,
mitochondrion
(
GO:0005739
)
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
X scale
0.108333 micrometer/pixel
Y scale
0.108333 micrometer/pixel
Z scale
-
T scale
30 seconds per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Satoh AO, et. al. Cell Rep. 2023 Mar 28;42(3):112229.
Related paper(s)
Contact
Yusuke Ohba
, Hokkaido University
, Department of Cell Physiology
Contributors
Aya O. Satoh, Yoichiro Fujioka
OMERO Project
Source