Detail of FigS4_Right-OCT_1
Project
Title
Images of OCT (optical coherence tomography) 1M, 3M, and 5M after transplantation of monkey iPSC-retina on right eye.
Description
Images of OCT (optical coherence tomography) 1 month, 3 months, and 5 months after transplantation of monkey iPSC-retina (induced pluripotent stem cell-derived retina). The monkey iPSC-retina was transplanted into an MHC-mismatched monkey right eye after focal laser outer nuclear layer ablation.
Release, Updated
2024-12-14
License
CC-BY
Kind
Image data
File Formats
.jpg
Data size
1.4 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
retina homeostasis
(
GO:0001895
)
Cellular Component (CC)
Biological Imaging Method
OCT
(
Fbbi:00000371
)
X scale
-
Y scale
-
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
See details in Uyama H, et. al. Stem Cell Reports. 2022 Nov 8;17(11):2392-2408.
Related paper(s)
Hirofumi Uyama, Hung-Ya Tu, Sunao Sugita, Suguru Yamasaki, Yasuo Kurimoto, Take Matsuyama, Takashi Shiina, Takehito Watanabe, Masayo Takahashi, Michiko Mandai (2022) Competency of iPSC-derived retinas in MHC-mismatched transplantation in non-human primates., Stem cell reports
Published in 2022 Oct 18 (Electronic publication in Oct. 18, 2022, midnight )
- doi: 10.1016/j.stemcr.2022.09.014
-
pmid: 36306783
(Abstract) Transplantation of embryonic/induced pluripotent stem cell-derived retina (ESC/iPSC-retina) restores host retinal ganglion cell light responses in end-stage retinal degeneration models with host-graft synapse formation. We studied the immunological features of iPSC-retina transplantation using major histocompatibility complex (MHC)-homozygote monkey iPSC-retinas in monkeys with laser-induced retinal degeneration in MHC-matched and -mismatched transplantation. MHC-mismatched transplantation without immune suppression showed no evident clinical signs of rejection and histologically showed graft maturation without lymphocytic infiltration, although immunological tests using peripheral blood monocytes suggested subclinical rejection in three of four MHC-mismatched monkeys. Although extensive photoreceptor rosette formation was observed on histology, evaluation of functional integration using mouse models such as mouse ESC-retina (C57BL/6) transplanted into rd1(C3H/HeJ, MHC-mismatched model) elicited light responses in the host retinal ganglion cells after transplantation but with less responsiveness than that in rd1-2J mice (C57BL/6, MHC-matched model). These results suggest the reasonable use of ESC/iPSC-retina in MHC-mismatched transplantation, albeit with caution.
Contact
Hirofumi Uyama, Michiko Mandai
, Kobe City Eye Hospital, Kobe City Eye Hospital
, Department of Ophthalmology , Department of Ophthalmology
Contributors
Hirofumi Uyama , Hung-Ya Tu , Sunao Sugita , Suguru Yamasaki , Yasuo Kurimoto, Take Matsuyama, Takehito Watanabe, Michiko Mandai
OMERO Project