Detail of Fig5D_MovieS2_FucciCA5
Project
Title
Time-lapse image of Fucci(CA)5 fluorescent protein in live HeLa cells
Description
Time-lapse image of Fucci(CA)5 fluorescent protein in live HeLa cells. In this Fucci(CA)5 system, we can monitor the cell-cycle progression by visualising red, green and merged color.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
252.9 MB
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain(s)
-
Cell Line
HeLa/Fucci(CA)5 (clone #16) (RCB 4919)
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell cycle phase transition
(
GO:0044770
)
Cellular Component (CC)
Biological Imaging Method
X scale
0.65 micrometer/pixel
Y scale
0.65 micrometer/pixel
Z scale
-
T scale
17 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Ando R, et. al. Cell Struct Funct. 2023 Jul 29;48(2):135-144
Related paper(s)
Ryoko Ando, Asako Sakaue-Sawano, Keiko Shoda, Atsushi Miyawaki (2023) Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression., Cell structure and function
Published in 2023 Jun 30 (Electronic publication in June 30, 2023, midnight )
- doi: 10.1247/csf.23028
-
pmid: 37394513
(Abstract) We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright green fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided more reliable nuclear labeling for monitoring cell-cycle progression than the 1(st) and 2(nd) generations that used mAG/mKO2 and mVenus/mCherry, respectively. Key Words: fluorescent protein, cell cycle, time-lapse imaging, flow cytometry.
Contact
Asako Sakaue-Sawano, Atsushi Miyawaki
, RIKEN, RIKEN
, Center for Brain Science , Center for Brain Science
, Laboratory for Cell Function Dynamics , Laboratory for Cell Function Dynamics
Contributors
OMERO Project
Source