Detail of Figure5C_Luc2_Ascl1
Project
SSBD:Repository
Title
Bioluminescent time-lapse image of Ascl1-Luciferse reporter mice's brain slices after iPaD virus injection
Description
Dentate gyrus of 11-month-old luciferase2 - Ascl1 reporter transgenic mice was injected by inducing Plagl2 and anti Dyrk1a (iPaD) - mCherryNLS lentivrus. iPaD - mCherryNLS expressed mainly at adult neural stem cells (NSCs) of dentate gyrus enhancing the proliferation of NSCs where Ascl1 supposed to express in an oscillatory manner. 4 week after iPaD lentivirus injection, brain slices containg dentate gyrus were exracted and placed in a gel supplemented by culture medium. Time-lapse imaging of the bioluminescent signal derived from Luciferse2 - Ascl1 reporter signal derived from lentivirus infected cells were detected
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
121.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neurogenesis
(
GO:0022008
)
,
stem cell differentiation
(
GO:0048863
)
Cellular Component (CC)
Biological Imaging Method
X scale
-
Y scale
-
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Kaise T, et. al. Genes Dev. 2022 Jan 1;36(1-2):23-37
Related paper(s)
Contact
Ryoichiro Kageyama
, RIKEN
, RIKEN Center for Brain Science
Contributors
Risa Sueda
OMERO Project