Detail of FigS13E_switch1503
Project
SSBD:Repository
Title
Photoswitchable SRS imaging of fixed HeLa cells stained by DAE620-Mito observed at the Raman on-resonance wave number
Description
Photoswitchable SRS (stimulated Raman scattering) signal of fixed HeLa cells stained with DAE620-Mito (triphenylphosphonium (Mito-tag) into DAE620 via a butyl linker) observed at the Raman on-resonance wave number (1503 cm^{-1}). The Raman probe was switched-on with 375 nm light and switched-off with 640 nm light.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
1.9 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
(
GO:0005739
)
Biological Imaging Method
inelastic scattering of photons
(
Fbbi:00000589
)
reversible saturable optical Raman transitions (RESORT) microscopy
reversible saturable optical Raman transitions (RESORT) microscopy
X scale
0.16 micrometer/pixel
Y scale
0.16 micrometer/pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Shou J, et. al. Sci Adv. 2023 Jun 16;9(24):eade9118.
Related paper(s)
Contact
Yasuyuki Ozeki, Mako Kamiya
, The University of Tokyo, The University of Tokyo
, Department of Electrical Engineering and Information Systems, Graduate School of Medicine
Contributors
Jingwen Shou, Ayumi Komazawa, Yuusaku Wachi, Hiroyoshi Fujioka, Minoru Kawatani, Takaha Mizuguchi, Hikaru Akaboshi
OMERO Project