Detail of Fig3A_video2_ph20TxRed500_Bin2_FpLrT10_15CP_150Hr_4_Pos30

(Too many images for preview; see images in SSBD:OMERO Dataset)


Project
Title
Time-lapse images of YK0083 cells continued growth and division against mcherry-cat gene deletion.
Description
Time-lapse images of YK0083 cells continued growth and division against mcherry-cat gene deletion.
Release, Updated
2024-01-23
License
CC BY
Kind
Image data
File Formats
.tif
Data size
11.8 GB

Organism
Escherichia coli ( NCBI:txid562 )
Strain(s)
YK0083
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to xenobiotic stimulus ( GO:0009410 ) cell growth ( GO:0016049 ) cell division ( GO:0051301 )
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.13 micrometer/pixel
Y scale
0.13 micrometer/pixel
Z scale
-
T scale
2 minutes of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Koganezawa Y, et. al. (2022) Elife, May 10;11:e74486.
Related paper(s)

Yuta Koganezawa, Miki Umetani, Moritoshi Sato, Yuichi Wakamoto (2022) History-dependent physiological adaptation to lethal genetic modification under antibiotic exposure., eLife, Volume 11

Published in 2022 May 10 (Electronic publication in May 10, 2022, midnight )

(Abstract) Genetic modifications, such as gene deletion and mutations, could lead to significant changes in physiological states or even cell death. Bacterial cells can adapt to diverse external stresses, such as antibiotic exposure, but can they also adapt to detrimental genetic modification? To address this issue, we visualized the response of individual Escherichia coli cells to deletion of the antibiotic resistance gene under chloramphenicol (Cp) exposure, combining the light-inducible genetic recombination and microfluidic long-term single-cell tracking. We found that a significant fraction ( approximately 40%) of resistance-gene-deleted cells demonstrated a gradual restoration of growth and stably proliferated under continuous Cp exposure without the resistance gene. Such physiological adaptation to genetic modification was not observed when the deletion was introduced in 10 hr or more advance before Cp exposure. Resistance gene deletion under Cp exposure disrupted the stoichiometric balance of ribosomal large and small subunit proteins (RplS and RpsB). However, the balance was gradually recovered in the cell lineages with restored growth. These results demonstrate that bacterial cells can adapt even to lethal genetic modifications by plastically gaining physiological resistance. However, the access to the resistance states is limited by the environmental histories and the timings of genetic modification.
(MeSH Terms)

Contact
Yuichi Wakamoto , The University of Tokyo , Graduate School of Arts and Sciences , Department of Basic Science
Contributors

OMERO Dataset
OMERO Project
Source