Detail of 3_FigS3A_UV_20s
Project
Title
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 20s.
Description
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 20s.
Release, Updated
2023-07-20
License
CC BY
Kind
Image data
File Formats
.tif
Data size
198.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
determination of left/right symmetry
(
GO:0007368
)
detection of nodal flow
(
GO:0003127
)
Cellular Component (CC)
non-motile cilium
(
GO:0097730
)
Biological Imaging Method
Spinning disk confocal microscope with custom-built optical pathway
X scale
0.13 micrometer/pixel
Y scale
0.13 micrometer/pixel
Z scale
-
T scale
100 msec per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Katoh TA, et. al. Science. 2023 Jan 6;379(6627):66-71.
Related paper(s)
Takanobu A Katoh, Toshihiro Omori, Katsutoshi Mizuno, Xiaorei Sai, Katsura Minegishi, Yayoi Ikawa, Hiromi Nishimura, Takeshi Itabashi, Eriko Kajikawa, Sylvain Hiver, Atsuko H Iwane, Takuji Ishikawa, Yasushi Okada, Takayuki Nishizaka, Hiroshi Hamada (2023) Immotile cilia mechanically sense the direction of fluid flow for left-right determination., Science (New York, N.Y.), Volume 379, Number 6627, pp. 66-71
Published in 2023 Jan 6 (Electronic publication in Jan. 5, 2023, midnight )
- doi: 10.1126/science.abq8148
-
pmid: 36603091
(Abstract) Immotile cilia at the ventral node of mouse embryos are required for sensing leftward fluid flow that breaks left-right symmetry of the body. However, the flow-sensing mechanism has long remained elusive. In this work, we show that immotile cilia at the node undergo asymmetric deformation along the dorsoventral axis in response to the flow. Application of mechanical stimuli to immotile cilia by optical tweezers induced calcium ion transients and degradation of Dand5 messenger RNA (mRNA) in the targeted cells. The Pkd2 channel protein was preferentially localized to the dorsal side of immotile cilia, and calcium ion transients were preferentially induced by mechanical stimuli directed toward the ventral side. Our results uncover the biophysical mechanism by which immotile cilia at the node sense the direction of fluid flow.(MeSH Terms)
Contact
Takanobu A. Katoh, Toshihiro Omori, Hiroshi Hamada
, The University of Tokyo, Tohoku University, Tata Instituite for Foundamental Research
, Graduate School of Medicine, Graduate School of Biomedical Engineering, National Center for Biological Science
, Cell Biology
Contributors
OMERO Project
Source