Detail of Fig4a_191013_01_INCIDER-1_EGTA
Project
Title
Time-lapse imaging of reversible intercellular NCad interactions by INCIDER with 10 mM EGTA in HEK293T cells.
Description
Time-lapse imaging of reversible intercellular NCad interactions by INCIDER with 10 mM EGTA in HEK293T cells.
Release, Updated
2023-07-20
License
CC-BY
Kind
Image data
File Formats
.oib
Data size
12.7 MB
Datatype
-
Molecular Function (MF)
cell-cell adhesion mediator activity
(
GO:0098632
)
cadherin binding involved in cell-cell adhesion
(
GO:0098641
)
Biological Process (BP)
cell adhesion
(
GO:0098602
)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
(
Fbbi:00000249
)
X scale
0.258 micrometer/pixel
Y scale
0.258 micrometer/pixel
Z scale
-
T scale
30 minutes of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Kanadome T, et. al. (2022) Commun Biol. Oct 7;5(1):1065.
Related paper(s)
Takashi Kanadome, Kanehiro Hayashi, Yusuke Seto, Mototsugu Eiraku, Kazunori Nakajima, Takeharu Nagai, Tomoki Matsuda (2022) Development of intensiometric indicators for visualizing N-cadherin interaction across cells., Communications biology, Volume 5, Number 1, pp. 1065
Published in 2022 Oct 7 (Electronic publication in Oct. 7, 2022, midnight )
- doi: 10.1038/s42003-022-04023-2
-
pmid: 36207396
(Abstract) N-cadherin (NCad) is a classical cadherin that mediates cell-cell interactions in a Ca(2+)-dependent manner. NCad participates in various biological processes, from ontogenesis to higher brain functions, though the visualization of NCad interactions in living cells remains limited. Here, we present intensiometric NCad interaction indicators, named INCIDERs, that utilize dimerization-dependent fluorescent proteins. INCIDERs successfully visualize reversible NCad interactions across cells. Compared to FRET-based indicators, INCIDERs have a ~70-fold higher signal contrast, enabling clear identification of NCad interactions. In primary neuronal cells, NCad interactions are visualized between closely apposed processes. Furthermore, visualization of NCad interaction at cell adhesion sites in dense cell populations is achieved by two-photon microscopy. INCIDERs are useful tools in the spatiotemporal investigation of NCad interactions across cells; future research should evaluate the potential of INCIDERs in mapping complex three-dimensional architectures in multi-cellular systems.(MeSH Terms)
Contact
Tomoki Matsuda
, Osaka University
, SANKEN (The Institute of Scientific and Industrial Research)
, Department of Biomolecular Science and Engineering
Contributors
OMERO Project
Source