Detail of Fig5F_HeLaS3_Hismet-HP1aCSD
Project
Title
Time-course FRET imaging of the nuclei of HeLa S3 cells transiently transfected with Hismet-HP1aCSD.
Description
Time-course FRET imaging of the nuclei of HeLa S3 cells transiently transfected with Hismet-HP1aCSD.
Release, Updated
2023-07-20,
2023-08-02
2023-08-02
Errata in Datasets
Added pseudo color images for "Intensity Modulated Display"
License
CC BY
Kind
Image data
File Formats
.tif
Data size
38.2 MB
Datatype
-
Molecular Function (MF)
H3K9me3 modified histone binding
(
GO:0062072
)
Biological Process (BP)
histone H3-K9 trimethylation
(
GO:0036124
)
Cellular Component (CC)
nucleus
(
GO:0005634
)
nucleosome
(
GO:0000786
)
Biological Imaging Method
FRET
(
Fbbi:00000367
)
X scale
0.1612 micrometer/pixel
Y scale
0.1612 micrometer/pixel
Z scale
-
T scale
900 second of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Sasaki K, et. al. (2022) Cell Chem Biol, Jul 21;29(7):1153-1161.e5.
Related paper(s)
Kazuki Sasaki, Michihiro Suzuki, Takeshi Sonoda, Tilman Schneider-Poetsch, Akihiro Ito, Motoki Takagi, Shinya Fujishiro, Yoshihiro Sohtome, Kosuke Dodo, Takashi Umehara, Hiroyuki Aburatani, Kazuo Shin-Ya, Yoichi Nakao, Mikiko Sodeoka, Minoru Yoshida (2022) Visualization of the dynamic interaction between nucleosomal histone H3K9 tri-methylation and HP1alpha chromodomain in living cells., Cell chemical biology, Volume 29, Number 7, pp. 1153-1161.e5
Published in 2022 Jul 21 (Electronic publication in June 20, 2022, midnight )
- doi: 10.1016/j.chembiol.2022.05.006
-
pmid: 35728598
(Abstract) Histone lysine methylation is an epigenetic mark that can control gene expression. In particular, H3K9me3 contributes to transcriptional repression by regulating chromatin structure. Successful mitotic progression requires correct timing of chromatin structure changes, including epigenetic marks. However, spatiotemporal information on histone modifications in living cells remains limited. In this study, we created an FRET-based probe for live-cell imaging based on the HP1alpha chromodomain (HP1alphaCD), which binds to H3K9me3. The probe was incorporated into chromatin and the emission ratio decreased after treatment with histone methyltransferase inhibitors, indicating that it successfully traced dynamic changes in H3K9me3. Upon entry into mitosis, the probe's emission ratio transiently increased with a concomitant increase in H3K9me3, then exhibited a stepwise decrease, probably due to loss of HP1alphaCD binding caused by phosphorylation of H3S10 and demethylation of H3K9me3. This probe will be a useful tool for detecting dynamic changes in chromatin structure associated with HP1alpha.(MeSH Terms)
Contact
Kazuki Sasaki, Minoru Yoshida
, RIKEN, RIKEN
, Center for Sustainable Resource Science, Center for Sustainable Resource Science
, Chemical Genomics Research Group, Chemical Genomics Research Group
Contributors
Kazuki Sasaki
OMERO Project
Source