Detail of Fig2c_CROPset
Project
Title
Time-course images of HeLa cells dynamics with fluorescence proteins vgCam and ATeam1.03.
Description
Time-course images of HeLa cells dynamics with fluorescence proteins vgCam and ATeam1.03.
Release, Updated
2023-07-20
License
CC-BY
Kind
Image data
File Formats
.tif
Data size
202.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
0.201 micrometer/pixel
Y scale
0.201 micrometer/pixel
Z scale
-
T scale
15 seconds of time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Sugiura K, et. al. (2022) Commun Biol. Nov 3;5(1):1172.
Related paper(s)
Kazunori Sugiura, Takeharu Nagai (2022) Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application., Communications Biology, Volume 5
Published in 2022 (Electronic publication in Nov. 3, 2022, midnight )
- doi: 10.1038/s42003-022-04153-7
-
pmid: 36329112
(Abstract) To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of wavelength properties of fluorescent proteins is underway. However, the shortest emission wavelength of fluorescent proteins has not been updated for more than 10 years. Here, we report the development of Sumire, a fluorescent protein emitting 414 nm violet fluorescence from a hydrated chromophore. The Sumire’s fluorescence property allows for the creation of FRET probes that can be used simultaneously with CFP-YFP based FRET probes for multi-parameter analysis.
Contact
Takeharu Nagai
, Osaka University
, SANKEN (The Institue of Scientific and Industrial Research)
Contributors
OMERO Project
Source