Detail of Fig2E_PKA_200626_008
Project
Title
Time-lapse images of the PKA activity in the transgenic mice.
Description
Time-lapse images of the PKA activity in the transgenic mice.
Release, Updated
2023-02-16
License
CC BY
Kind
Image data
File Formats
.oib
Data size
20.9 MB
Protein names
PKA
Datatype
-
Molecular Function (MF)
Rho-dependent protein serine/threonine kinase activity
(
GO:0072518
)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
(
Fbbi:00000249
)
X scale
0.284 micrometer/pixel
Y scale
0.284 micrometer/pixel
Z scale
1 micrometer/slice
T scale
6.64 seconds per frame
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Imanishi A, et. al. (2021) Am J Pathol, Jan;191(1):194-203.
Related paper(s)
Ayako Imanishi, Hiroshi Ichise, Chuyun Fan, Yasuaki Nakagawa, Koichiro Kuwahara, Kenta Sumiyama, Michiyuki Matsuda, Kenta Terai (2021) Visualization of Spatially-Controlled Vasospasm by Sympathetic Nerve-Mediated ROCK Activation., The American journal of pathology, Volume 191, Number 1, pp. 194-203
Published in 2021 Jan (Electronic publication in Oct. 16, 2020, midnight )
- doi: 10.1016/j.ajpath.2020.09.012
-
pmid: 33069718
(Abstract) Contraction of vascular smooth muscle is regulated primarily by calcium concentration and secondarily by ROCK activity within the cells. In contrast to the wealth of information regarding regulation of calcium concentration, little is known about the spatiotemporal regulation of ROCK activity in live blood vessels. Here, we report ROCK activation in subcutaneous arterioles in a transgenic mouse line that expresses a genetically encoded ROCK biosensor based on the principle of Frster resonance energy transfer by two-photon excitation in vivo imaging. Rapid vasospasm was induced upon laser ablation of arterioles, concomitant with a transient increase in calcium concentration in arteriolar smooth muscles. Unlike the increase in calcium concentration, vasoconstriction and ROCK activation continued for several minutes after irradiation. Both the ROCK inhibitor, fasudil, and the ganglionic nicotinic acetylcholine receptor blocker, hexamethonium, inhibited laser-induced ROCK activation and reduced the duration of vasospasm at the segments distant from the irradiated point. These observations suggest that vasoconstriction is initially triggered by a rapid surge of cytoplasmic calcium and then maintained by sympathetic nerve-mediated ROCK activation.(MeSH Terms)
- Animals
- Autonomic Nervous System/physiology
- Calcium Signaling/physiology
- Fluorescence Resonance Energy Transfer
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Muscle, Smooth, Vascular/enzymology[major]
- Muscle, Smooth, Vascular/innervation
- Vasoconstriction/physiology[major]
- rho-Associated Kinases/metabolism[major]
Contact
Kenta Terai
, kyoto University
, Graduate School of Medicine
, Department of Pathology and Biology of Diseases
Contributors
OMERO Project
Source