Detail of Fig1a_MI_Aging
Project
SSBD:Repository
Title
Time-lapse microscopy images of oocytes expressing 2mEGFP-CENP-C and H2B-mCherry in a aged (16 months old) BDF1 mouse
Description
NA
Release, Updated
2017-10-03,
2018-11-15
2018-11-15
License
CC BY-NC-SA
Kind
Image data
based on Experiment
File Formats
Data size
1.8 GB
Protein names
CENP, H2B
Protein tags
2mEGFP, mCherry
Datatype
kinetochore dynamics
Molecular Function (MF)
Biological Process (BP)
meiosis I
(
GO:0007127
)
Cellular Component (CC)
kinetochore
(
GO:0000776
)
,
chromosome
(
GO:0005694
)
Biological Imaging Method
XYZ Scale
XY: 0.1186094 micrometer/pixel, Z: 1.5 micrometer/frame
T scale
3 minute for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Zeiss LSM710
Detector model
-
Objective model
Zeiss C-Apochromat 40x/1.2NA W
Filter set
-
Summary of Methods
See details in Sakakibara et al. (2015) Nat Commun. 2013; 4:2212.
Related paper(s)
Contact
Tomoya Kitajima
, RIKEN
, Center for Developmental Biology
, Laboratory for Chromosome Segregation
Contributors
Yogo Sakakibara, Tomoya Kitajima
OMERO Dataset
OMERO Project