SSBD:database

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Detail of pmPAR-2

Project

SSBD:Repository

Title

BDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::phospho-mimicPAR-2 in a C. elegans wild-type embryo

Description

Release, Updated

2017-10-03,
2020-02-03

License

CC BY

Kind

Quantitative data based on Experiment

File Formats

Data size

5.3 MB

Organism

Caenorhabditis elegans ( NCBI:txid6239 )

Strain(s)

Cell Line

Datatype

single molecule dynamics

Molecular Function (MF)

Biological Process (BP)

cellular protein localization ( GO:0034613 )

Cellular Component (CC)

Biological Imaging Method

XYZ Scale

XY: 0.0667 micrometer/pixel, Z: NA

T scale

0.05 second for each time interval

Image Acquisition

Experiment type

TimeLapse

Microscope type

ConfocalMicroscope

Acquisition mode

TIRF

Contrast method

Fluorescence

Microscope model

Nikon TE2000

Detector model

Hamamatsu Photonics ImagEM

Objective model

Nikon Apo TIRF 60X/1.49

Filter set

Summary of Methods

The original move of pmPAR-2 (tiff format) is rotated at 135 degree and processed through Rolling Ball and Kalman filters to obtain pmPAR-2_RBKL.avi (Movie S3). The PAR-2 trajectories (xlsx file) were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.