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Detail of wtPAR-2


Project
SSBD:Repository
Title
BDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::PAR-2 expressed in a C. elegans wild-type embryo
Description
NA
Release, Updated
2017-10-03,
2020-02-03
License
CC BY
Kind
Quantitative data based on Experiment
File Formats
Data size
5.8 MB

Organism
Caenorhabditis elegans ( NCBI:txid6239 )
Strain(s)
-
Cell Line
-

Datatype
single molecule dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization ( GO:0034613 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.0667 micrometer/pixel, Z: NA
T scale
0.05 second for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
TIRF
Contrast method
Fluorescence
Microscope model
Nikon TE2000
Detector model
Hamamatsu Photonics ImagEM
Objective model
Nikon Apo TIRF 60X/1.49
Filter set

Summary of Methods
The original move of wtPAR-2 (tiff format) is rotated at 45 degree and processed through Rolling Ball and Kalman filters to obtain wtPAR-2_RBKL.avi (Movie S1). The PAR-2 trajectories were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.
Related paper(s)

Contact
Yukinobu Arata, Yasushi Sako , RIKEN , Wako , Cellular Informatics Laboratory
Contributors
Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul W. Wiseman, Hitoshi Sawa, Tetsuya J. Kobayashi, Hugo B. Brandão, Tatsuo Shibata, Yasushi Sako

Local ID
wtPAR-2
BDML ID
f870c62c-97e6-4fa1-9bb6-7290c911e7b8
BDML/BD5
External Link
Source