Summary of 92-Uda-CellsigDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
21 ( Image datasets: 21, Quantitative data datasets: 0 )
Size of Datasets
1.0 GB ( Image datasets: 1.0 GB, Quantitative data datasets: 0 bytes )

Organism(s)
H. sapiens
Strain(s)
HeLa
Gene symbol(s)
NA
Protein name(s)
ERK, HRasCT, PHFF, PIF3, PIF6

Datatype
cell signaling dynamics
Molecular Function (MF)
Biological Process (BP)
intracellular signal transduction
Cellular Component (CC)
cell
Biological Imaging Method
-
XYZ Scale
XY:0.4919990 micrometer/pixel, Z:1 micrometer/slice, XY:0.331 micrometer/pixel, Z:1 micrometer/slice
T scale
3.67 second for each time interval, 3.74 second for each time interval, 4.08 second for each time interval, 3.48 second for each time interval, 2.62 second for each time interval, 2.63 second for each time interval, 2.60 second for each time interval, 4 second for each time interval, 3.76 second for each time interval, 3.64 second for each time interval, 3.59 second for each time interval, 2.59 second for each time interval, 3.61 second for each time interval, 3.55 second for each time interval, 2.68 second for each time interval, 120 second for each time interval, 2.55 second for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus IX81 FV1000
Detector model
-
Objective model
Olympus UPLANSAPO 60xO/N.A. 1.35
Filter set
-

Related paper(s)

Youichi Uda, Yuhei Goto, Shigekazu Oda, Takayuki Kohchi, Michiyuki Matsuda, Kazuhiro Aoki (2017) Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling., Proceedings of the National Academy of Sciences of the United States of America, Volume 114, Number 45, pp. 11962-11967

Published in 2017 Nov 7 (Electronic publication in Oct. 24, 2017, midnight )

(Abstract) Optogenetics is a powerful tool to precisely manipulate cell signaling in space and time. For example, protein activity can be regulated by several light-induced dimerization (LID) systems. Among them, the phytochrome B (PhyB)-phytochrome-interacting factor (PIF) system is the only available LID system controlled by red and far-red lights. However, the PhyB-PIF system requires phycocyanobilin (PCB) or phytochromobilin as a chromophore, which must be artificially added to mammalian cells. Here, we report an expression vector that coexpresses HO1 and PcyA with Ferredoxin and Ferredoxin-NADP+ reductase for the efficient synthesis of PCB in the mitochondria of mammalian cells. An even higher intracellular PCB concentration was achieved by the depletion of biliverdin reductase A, which degrades PCB. The PCB synthesis and PhyB-PIF systems allowed us to optogenetically regulate intracellular signaling without any external supply of chromophores. Thus, we have provided a practical method for developing a fully genetically encoded PhyB-PIF system, which paves the way for its application to a living animal.
(MeSH Terms)

Contact
Kazuhiro Aoki , National Institutes of Natural Sciences , National Institute for Basic Biology , Division of Quantitative Biology
Contributors
Youichi Uda, Yuhei Goto, Shigekazu Oda, Takayuki Kohchi, Michiyuki Matsuda, Kazuhiro Aoki


Dataset List of 92-Uda-CellsigDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 4189
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4190
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4191
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4192
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4193
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4194
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4195
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4196
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4197
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4198
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4199
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4200
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4201
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
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# 4202
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4203
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4204
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4205
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4206
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4207
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4208
Dataset Kind Image data
Dataset Size 39.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4209
Datast ID fig4E
Dataset Kind Image data
Dataset Size 38.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data